Oil Palm Leaf Sampling Method
Samples were taken in the morning due to low level
sunlight exposure to the leaflet of oil palm. Front 17 detected from fully open
frond at terminal shoot which is the frond 1 where on the left or right of the
shoot depending on the left handed and right handed harvesting pattern. Frond
17 was identified by counting downwards from the Frond 1 which was the fully
open frond at the terminal shoot.The frond was removed from the tree by using a sickle
by cutting three quarters of the total frond length. Frond 17 was identified by
the presence of the thorn that was located at three quarter length cut off
frond. The frond
was cut into three (3) approximately equal sections and whereby the tip and
base sections were discarded and placed in the frond placement/stacking. The 12
leaflets were removed from the frond by using sharp knife which consisted of
six leaflets removed from the midpoint of the frond section with three from
each side of the frond. The remaining included three leaflets from the upper
rank of the frond section and three leaflets from the lower rank of the fronds. The leaflets were stored in a cool place
at Natural Product Research and Development Centre Laboratory. Then, each
leaflet sample was dry cleaned up and not washed to remove particles of dust
and small insect that might affect the result. After cleaning, six leaflets
from 12 leaflets that collected were used for determination of leaf area and
for measurement by using AutoCAD
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